access array loading reagent (fluidigm)

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fluidigm access array loading reagent
Access Array Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/access+array+loading+reagent/Access+Array+Loading+Reagent/us12404547-446-56-60
Average 90 stars, based on 196 article reviews

access array loading reagent - by Bioz Stars, 2026-09

90/100 stars

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Amplification:

Article Title: Methods and systems for monitoring a recipient of an allograft
Article Snippet: .. Materials to be used for this amplification protocol include a Fluidigm Access Array, or chip, a high-fidelity polymerase such as FastStart High Fidelity (Roche), Expand High Fidelity (Roche), Phusion Flash II (ThermoFisher Scientific), Phusion Hot Start II (ThermoFisher Scientific), KAPA HiFi (Kapa BioSystems), or KAPA2G (Kapa Biosystems), 1× Access Array Harvest Solution (Fluidigm, PN 100-1031), 20× Access Array Loading reagent (Fluidigm), and the 226 primer pairs designed as described above. ..

Article Title: Methods and systems for monitoring a recipient of an allograft
Article Snippet: .. Materials used for this amplification protocol included a Fluidigm Access Array, or chip, the high-fidelity DNA polymerase FastStart High Fidelity (Roche), 1× Access Array Harvest Solution (Fluidigm, PN 100-1031), 20× Access Array Loading reagent (Fluidigm), and the 266 primer pairs designed as described above. ..

Article Title: Methods and systems for monitoring a recipient of an allograft
Article Snippet: DNA Amplification The cell-free DNA from the pre-amplification was amplified according to the Fluidigm Access Array process and protocols (See page 63 of Access Array System for Illumina Sequencing System). .. Materials used for this amplification protocol included a Fluidigm Access Array, or chip, the high-fidelity DNA polymerase Phusion Flash II (ThermoFisher Scientific), 1× Access Array Harvest Solution (Fluidigm, PN 100-1031), 20× Access Array Loading reagent (Fluidigm), and the 266 primer pairs designed as described above. .. Instruments used for this amplification protocol included two IFC Controller AX (Fluidigm) and one FC1 cycler (Fluidigm).

Article Title: Methods of monitoring immunosuppressive therapies in a transplant recipient
Article Snippet: DNA Amplification The cell-free DNA from the pre-amplification was amplified according to the Fluidigm Access Array process and protocols (See page 63 of Access Array System for Illumina Sequencing System). .. Materials used for this amplification protocol included a Fluidigm Access Array, or chip, the high-fidelity DNA polymerase FastStart High Fidelity (Roche), 1X Access Array Harvest Solution (Fluidigm, PN 100-1031), 20X Access Array Loading reagent (Fluidigm), and the 266 primer pairs designed as described above. .. Instruments used for this amplification protocol included two IFC Controller AX (Fluidigm) and one FC1 cycler (Fluidigm).

Article Title: Methods and systems for monitoring a recipient of an allograft
Article Snippet: DNA Amplification The cell-free DNA is amplified according to the Fluidigm Access Array process and protocols (See page 63 of Access Array System for Illumina Sequencing System). .. Materials to be used for this amplification protocol include a Fluidigm Access Array, or chip, FastStart High Fidelity PCR system (Roche), 1× Access Array Harvest Solution (Fluidigm, PN 100-1031), 20× Access Array Loading reagent (Fluidigm), and the 92 primer pairs designed as described above. .. Instruments to be used for this amplification protocol include two IFC Controller AX (Fluidigm) and one FC1 cycler (Fluidigm).

Biomarker Discovery:

Article Title: Deciphering the evolution of the temporal and geographic distribution of French Mycobacterium bovis genotypes using a high throughput SNP-targeted amplicon sequencing method.
Article Snippet: .. The validation master mix solution with final concentrations contained: 1× FastStart High Fidelity Reaction Buffer without MgCl2 (Roche, Meylan, France), 4.5 mM MgCl2 (Roche), 5% DMSO (Roche), 200 μM each PCR grade Nucleotide Mix (Roche), 1× Access Array loading reagent (Fluidigm, San Francisco, USA), tagged TSF and TSR primers (200 nM each), 1 μl of DNA (lysates), 0.05 U/μl FastStart High Fidelity Enzyme Blend (Roche), molecular grade water to 20 μl final volume. .. The Thermocycler program run with 35 cycles was performed as recommended in the Fluidigm targeted sequencing primer validation protocol (PN 100–3770 H1) and provided in Table S4.

Polymerase Chain Reaction:

Article Title: Extensive allopolyploidy in the neotropical genus Lachemilla (Rosaceae) revealed by PCR-based target enrichment of the nuclear ribosomal DNA cistron and plastid phylogenomics.
Article Snippet: Validation reactions simulate the four- primer reaction of the Fluidigm microfluidic PCR system using a standard thermocycler. .. Reactions were carried out in a final volume of 10 μL with final concentrations of 1× FastStart High Fidelity Reaction Buffer without MgCl2 (Roche, Mannheim, Germany), 4.5 mM MgCl2 (Roche), 5% DMSO (Roche), 200 μM ea PCR Grade Nucleotide Mix (Roche), 0.05 U/μL, FastStart High Fidelity Enzyme Blend (Roche), 1× Access Array Loading Reagent (Fluidigm), 400 nM Access Array Barcode Primers for Illumina, 30–70 ng/μL genomic DNA, 50 mM of each target- specific primer and PCR Certified Water (Teknova, Hollister, CA, USA) to volume. .. Amplification conditions consisted of 50°C for 2 min, 70°C for 20 min, 95°C for 10 min; 10 cycles of 95°C 15 for s, 60°C for 30 s, and 72°C for 1 min; two cycles of 95°C for 15 s, 80°C for 30 s, 60°C for 30 s, and 72°C for 1 min; eight cycles of 95°C for 15 s, 60°C for 30 s, and 72°C for 1 min; two cycles of 95°C for 15 s, 80°C for 30 s, 60°C for 30 s, and 72°C for 1 min; eight cycles of 95°C for 15 s, 60°C for 30 s, and 72°C for 1 min; and 5 cycles of 95°C for 15 s, 80°C for 30 s, 60°C for 30 s, and 72°C for 1 min. Amplicons from these reactions were analyzed in a QIAxcel Advance System (Qiagen, Valencia, CA, USA), and primer pairs that produced a single amplicon and had no (or minimal) primer dimers were selected.

Article Title: Prenatal food insecurity post Hurricane Maria is associated with decreased Veillonella in the infant gut
Article Snippet: .. The reaction mix consisted of 1X High Fidelity FastStart Reaction Buffer without MgCl2 (Roche), 4.5nM MgCl2 (Roche), 5% DMSO (Roche), 200uM PCR Grade Nucleotide Mix (Roche), 0.05 U/μL 5 U/μL FastStart High Fidelity Enzyme Blend (Roche), 1X Access Array Loading Reagent (Fluidigm), 1ul DNA, and water. ..

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